Microscopy and Cell Biology: New Methods and New Questions. Analytical and Bioanalytical Chemistry 2019, 411 Fluctuation correlation spectroscopy and its applications in homogeneous analysis. Di Su, Yuemei Hou, Chaoqing Dong, Jicun Ren.Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification. Florian Stehr, Johannes Stein, Florian Schueder, Petra Schwille, Ralf Jungmann.A diffusiophoretic mechanism for ATP-driven transport without motor proteins. Beatrice Ramm, Andriy Goychuk, Alena Khmelinskaia, Philipp Blumhardt, Hiromune Eto, Kristina A.Bulletin of the Korean Chemical Society 2021, 42 Ultrasensitive Capsaicin Sensor Based on Endogenous SingleвЂђMolecule Fluorophore Enhancement and Quenching Interface on Gold Nanoislands. Suresh Kumar Chakkarapani, Seungah Lee, Seong Ho Kang.Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters. Johannes Stein, Florian Stehr, Ralf Jungmann, Petra Schwille.Programming Temporal DNA Barcodes for Single-Molecule Fingerprinting. A High-Throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates. BrandГЈo, Paul FranГ§ois, Gonzalo Cosa, Paul W. Simon Sehayek, Yasser Gidi, Viktorija Glembockyte, Hugo B.Toward Absolute Molecular Numbers in DNA-PAINT. Johannes Stein, Florian Stehr, Patrick Schueler, Philipp Blumhardt, Florian Schueder, Jonas MГјcksch, Ralf Jungmann, Petra Schwille.The Journal of Physical Chemistry C 2019, 123 Discriminating Single Base Pair Mismatches in DNA Using Glutathione-Templated Copper Nanoclusters.

Srikrishna Pramanik, Laxmikanta Khamari, Somen Nandi, Saptarshi Mukherjee.In Vivo Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow. The Journal of Physical Chemistry Letters 2021, 12 Differentiating a Least-Stable Single Nucleotide Mismatch in DNA Via Metal Ion-Mediated Base Pairing and Using Thioflavin T as an Extrinsic Fluorophore. Srikrishna Pramanik, Laxmikanta Khamari, Saptarshi Mukherjee.Monochromatic Fluorescent Barcodes Hierarchically Assembled from Modular DNA Origami Nanorods. Victor Pan, Wen Wang, Ian Heaven, Tanxi Bai, Yongxin Cheng, Chunlai Chen, Yonggang Ke, Bryan Wei.This article is cited by 20 publications. We varied the number of base pairs in the duplex, yielding different binding times in the range of hundreds of milliseconds to tens of seconds, allowing us to quantify the respective surface affinities and binding rates. We chose the transient hybridization of fluorescently labeled single-stranded DNA to the complementary handles of surface-immobilized DNA origami structures as a reliable and well-characterized test system. In addition to providing a high surface selectivity, our new approach resolves association and dissociation rates in equilibrium over a wide range of time scales. Instead, we perform correlation analysis on a spatially integrated signal, effectively converting the illuminated surface area into the measurement volume. In contrast to most modern applications of TIR-FCS, we completely disregard spatial information in the lateral direction. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescence correlation spectroscopy with total internal reflection illumination (TIR-FCS) and camera-based detection. We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors.

Adding images in andor solis